Microbiology of water in production of food of animal origin

Authors

  • Bojan Golić Public Institution Veterinary Institute Republic of Srpska „Dr Vaso Butozan“, Banja Luka, Bosnia and Herzegovina
  • Tanja Ilić Public Institution Veterinary Institute Republic of Srpska „Dr Vaso Butozan“, Banja Luka, Bosnia and Herzegovina
  • Saša Lovrić Public Institution Veterinary Institute Republic of Srpska „Dr Vaso Butozan“, Banja Luka, Bosnia and Herzegovina
  • Dragan Knežević Public Institution Veterinary Institute Republic of Srpska „Dr Vaso Butozan“, Banja Luka, Bosnia and Herzegovina

DOI:

https://doi.org/10.63356/agrores.2025.019

Keywords:

water, food, animals, production, microbiology

Abstract

One of the most important prerequisites for safe food of animal origin is healthy animals used to produce food of animal origin. Water used in the production of food of animal origin must, in terms of its quality, including microbiological safety, meet the requirements relating to drinking water intended for human consumption. The experiment used drinking water originating from primary food production, including water from animal farms and meat, milk and fish production, sampled in 2023-2024 from area of the Republic of Srpska (Bosnia and Herzegovina). A total of 1,044 samples were examined (744 in 2023, and 300 in 2024). The aim of the study is to determine the microbiological status of drinking water from production of food af animal origin, using standard methods BAS EN ISO 6222, BAS EN ISO 7899-2 and BAS EN ISO 9308-1. Analyzing results that include all categories of tested water, the research determined an average of 55.08% satisfactory samples and 44.92% unsatisfactory samples in 2023, and 66.26% satisfactory samples and 33.74% unsatisfactory samples in 2024. A significantly higher number of unsatisfactory samples of well and spring water was observed compared to the water supply system. In relation to the tested parameters, 16.33% of samples were unsatisfactory due to an increased number of microorganisms cultivated at 22°C, and 22.49% of samples due to an increased number of microorganisms cultivated at 37°C. When it comes to pathogens, 11.16% of unsatisfactory samples were due to the presence of intestinal enterococci and 16.28% due to the presence of Escherichia coli and coliform. Considering that almost one third of water samples are unsatisfactory, and the significant presence of pathogenic bacteria, it is necessary to take more frequent and detailed measures for disinfection and water control, to ensure optimal prerequisites to produce food of animal origin.

References

Amaral, L. A., Nader Filho, A., Rossi Junior, O. D., & Penha, L. H. C. (1995). Características microbiológicas da água utilizada no processo obtenção do leite. Pesquisa Veterinária Brasileira, 15(2/3), 85-88.

Amenu, K. (2013). Assessment of water sources and quality for livestock and farmers in the Rift Valley area of Ethiopia: implications for health and food safety. Sierke Verlag.

Amenu, K., Shitu, D., & Abera, M. (2016). Microbial contamination of water intended for milk container washing in smallholder dairy farming and milk retailing houses in southern Ethiopia. Springerplus, 5(1), 1195.

Butcher, G. D., Jacob, J. P., & Mather, F. B. (1999). Common poultry diseases, Fact Sheet PS-47. Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, 1-19.

Dawson, D. (2000). Water Quality for the Food Industry: Management and Microbiological Issues. (Guideline No. 27). Campden and Chorleywood Food Research Association.

Denžić, M., Sokolović, J., Jaki-Tkalec, V., Majnarić, D., & Pavliček, D. (2013). Analysis of drinking water milk producers in the area of city Jastrebarsko. VII Scientific-professional conference Water and public water supply, Book of Abstracts, 139-140.

Directive 2020/2184, No. L435. On the quality of water intended for human consumption (recast). European Parliament and Council.

Edberg, S. C., Rice, E. W., Karlin, R. J., & Allen, M. J. (2000). Escherichia coli: the best biological drinking water indicator for public health protection. Journal of Applied Microbiology, 88, 106-116.

Fridrich, B., Krcmar, D., Dalmacija, B., Molnar, J., Pesic, V., Kragulj, M., & Varga N. (2014). Impact of wastewater from pig farm lagoons on the quality of local groundwater. Agricultural Water and Management, 135, 40-53.

Golić, B., Knežević, D., & Pećanac, B. (2023a). Assesment of the microbiological status of drinking water on animal farms. XIV International Scientific Agricultural Symposium „Agrosym 2023“, Proceedings, 1086-1093.

Golić, B., Knežević, D., & Pećanac, B. (2024). Microbiology of water in primary food production. XV International Scientific Agricultural Symposium „Agrosym 2024“, Proceedings, 514-519.

Golić, B., Nedić, D. N., & Pećanac, B. (2023b). Water in food industry – microbiological review. Veterinary Journal of Republic of Srpska, 23(1-2), 14-22.

Golić, B., Pećanac, B., & Knežević, D. (2023c). Evaluation of the microbiological suitability of drinking water on farms of domestic animals. XII International Symposium on Agricultural Sciences AgroRes 2023, Book of Proceedings, 301-316.

Habes, S., Hasanagic, E., Aldzic, A., Smjecanin, E., & Jukic, H. (2015). Microbiological quality of processed water during processing of milk and dairy products. Balkan Journal of Health Science, 3(2), 34-38.

Hammes, F., Berney, M., Wang, Y., Vital, M., Koster, O., & Egli, T. (2008). Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter for drinking water treatment processes. Water Research, 42, 269-277.

Hijnen, W. A. M., van Veenendaal, D. A., van der Speld, W. H. M., Visser, A., Hoogenboezem, W., & van der Kooij, D. (2000). Enumeration of faecal indicator bacteria in large volumes using in site membrane filtration to assess water treatment efficiency. Water Research, 34, 1659-1665.

Hoefel, D., Monis, P. T., Grooby, W. L., Andrews, S., & Saint, C. P. (2005). Profiling bacterial survival through a water treatment process and subsequent distribution system. Journal of Applied Microbiology, 99, 175-186.

Institute for standardization of Bosnia and Herzegovina. (2003a). Water quality - Enumeration of culturable microorganisms - Colony count by inoculation in a nutrient agar culture medium, BAS EN ISO 6222.

Institute for standardization of Bosnia and Herzegovina. (2003b). Water quality - Detection and enumeration of intestinal enterococci - Part 2: Membrane filtration method, BAS EN ISO 7899-2.

Institute for standardization of Bosnia and Herzegovina. (2018). Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora, BAS EN ISO 9308-1/A1.

Jaki, V., Majnarić, D., & Lukačić, M. (2010). The analysis of drinking water from the milk collection point in the Križevci area. 39th Croatian Symposium of Dairy Experts with International Participation, Book of Abstracts, 56-57.

Kalaba, V., Golić, B., & Ilić, T. (2020). Microbiological safety of water in primary production of food. Veterinary Journal of Republic of Srpska, 20(1-2), 66-80.

Kivaria, F. M., Noordhuizen, J. P. T. M., & Kapaga, A. M. (2006). Evaluation of the hygienic quality and associated public health hazards of raw milk marketed by smallholder dairy producers in the Dar es Salaam region, Tanzania. Tropical Animal Health and Production, 38(3), 185-194.

Maharjan, P., Clark, T., Kuenzel, C., Foy, M. K., & Watkins, S. (2016). On-farm monitoring of the impact of water system sanitation on microbial levels in broiler house water supplies. Journal of Applied Poultry Research, 25, 266-271.

Perkins, N. R., Kelton, D. F., Hand, K. J., MacNaughton, G., Berke, O., & Leslie, K. E. (2009). An analysis of the relationship between bulk tank milk quality and wash water quality on dairy farms in Ontario, Canada. Journal of Dairy Science, 92, 3714-3722.

Prest, E. I., Hammes, F., van Loosdrecht, M. C. M., & Vrouwenvelder, J. S. (2016). Biological Stability of Drinking Water: Controlling Factors, Methods, and Challenges. Frontiers in Microbiology, 7, 45.

Rulebook (2017). Pravilnik o zdravstvenoj ispravnosti vode za piće namijenjene za ljudsku upotrebu. (2017). Službeni Glasnik Republike Srpske, 88/17.

Sasakova, N., Veselitz-Lakticova, K., Hromada, R., Chvojka, D., Koscco, J., & Ondrasovic, M. (2013). Contamination of individual sources of drinking water located in environmentally polluted Central Spis Region (Slovakia). Journal of Microbiology, Biotechnology and Food Sciences, 3, 262-265.

World Health Organisation. (2008). Guidelines for Drinking-water Quality, 3rd ed., Vol. 1.

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Published

2025-05-23

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