The impact of sowing density on the productivity and growth dynamics of Ocimum basilicum grown as microgreens
DOI:
https://doi.org/10.63356/agrores.2025.001Keywords:
basil, germination, growth dynamics, vegetative development, fresh yieldAbstract
The increasing interest in fresh, functional, and nutraceutical products has driven the demand for healthy and longevity-promoting foods, with microgreens gaining popularity due to their vibrant colors, delicate texture, and rich phytonutrient content. Among various microgreens, Ocimum basilicum is widely recognized for its aromatic, bioactive properties, and nutritional composition, making it a promising functional food. This study aimed to investigate the impact of sowing density on the productivity, growth dynamics, and quality of O. basilicum cultivated as microgreens, focusing on improving production efficiency. The experiment was conducted in November 2024 in a controlled environment with three sowing densities: 2.5 g/m² (control, C), 3.5 g/m² (T1), and 4.5 g/m² (T2). Key parameters such as seed germination, plant height, leaf area, and fresh yield were evaluated throughout the growth cycle. Results showed that higher sowing densities (T2) positively affected seed germination and plant height during the initial growth stages. Although initial growth was more vigorous in the control treatment (C), the differences between treatments became less pronounced over time. The study concluded that optimizing sowing density is crucial for maximizing microgreens yield and improving the overall sustainability of basil production, providing valuable insights for commercial growers.
References
Adams, P. A., et al. (2016). Influence of various seed treatments on seedling establishment and early growth of Brassica napus. Agronomy Journal, 108(4), 1456-1463. DOI: 10.2134/agronj2015.0456
Chandra, S., Kim, Y., & Kim, S. (2012). Factors affecting the production and quality of microgreens. Horticulture, Environment, and Biotechnology, 53(6), 565-572. DOI: 10.1007/s13580-012-0173-2
Choe, U., Yu, L. L., & Wang, T. T. (2018). The science behind microgreens as an exciting new food for the 21st century. Journal of Agricultural and Food Chemistry, 66(44), 11519-11530. DOI: 10.1021/acs.jafc.8b03096
Fayezizadeh, M. R., Ansari, N. A., Sourestani, M. M., & Hasanuzzaman, M. (2023). Biochemical compounds, antioxidant capacity, leaf color profile and yield of basil (Ocimum sp.) microgreens in floating system. Plants, 12(14), 2652. DOI: 10.3390/plants12142652
Gupta, A., Sharma, T., Singh, S. P., Bhardwaj, A., Srivastava, D., & Kumar, R. (2023). Prospects of microgreens as budding living functional food: Breeding and biofortification through OMICS and other approaches for nutritional security. Frontiers in Genetics, 14, 1053810. DOI: 10.3389/fgene.2023.1053810
Kou, X., Shen, L., & Zhang, H. (2013). Microgreens: A review of the growth conditions, benefits, and challenges of this emerging crop. Horticultural Science & Technology, 31(3), 301-308.
Pinto, E., Almeida, M., Aguiar, A., & Ferreira, A. (2015). Nutritional content and bioactive compounds of microgreens. International Journal of Food Science & Technology, 50(4), 1053-1062. DOI: 10.1111/ijfs.12778
Radford, P. J. (1967). Growth analysis formulae‐their use and abuse. Crop Science, 7(3), 171-175.
Ramin, A. A. (2006). Effects of salinity and temperature on germination and seedling establishment of sweet basil (Ocimum basilicum L.). Journal of Herbs, Spices & Medicinal Plants, 11(4), 81-90.
Renna, M., Di Gioia, F., Leoni, B., Mininni, C., & Santamaria, P. (2017). Culinary assessment of self-produced microgreens as basic ingredients in sweet and savory dishes. Journal of Culinary Science & Technology, 15(2), 126-142. DOI: 10.1080/15428052.2016.1225538
Rizzo, G., & Baroni, L. (2018). Soy, soy foods and their role in vegetarian diets. Nutrients, 10(1), 43. DOI: 10.3390/nu10010043
Smith, M. J., et al. (2018). Role of seed priming in enhancing the growth and stress tolerance of crops. Journal of Plant Physiology, 223, 71-78. DOI: 10.1016/j.jplph.2018.02.00
Sun, X., Zhang, L., Liu, L., & Deng, S. (2013). Effect of harvest time on the microgreens of four species in the Brassicaceae family. Scientia Horticulturae, 159, 22-28. DOI: 10.1016/j.scienta.2013.04.032
Wang, J., et al. (2019). Effects of plant density and nitrogen levels on soybean yield and quality. Field Crops Research, 240, 105-114. DOI: 10.1016/j.fcr.2019.06.018
Xiao, Z., Lester, G. E., Luo, Y., & Wang, Q. (2012). Microgreens of Brassicaceae: Vitamin C, phenolics, carotenoids, and antioxidants. Journal of Food Science, 77(4), 464-C470. DOI: 10.1111/j.1750-3841.2012.02608.x
Xiao, Z., Lester, G. E., Luo, Y., & Wang, Q. (2015). A comparison of antioxidant activity and nutritional composition of 12 microgreens. Journal of Food Science, 80(4), 454-C460. DOI: 10.1111/1750-3841.12832
Zhang, Y., et al. (2021). Nutritional quality and health benefits of microgreens, a crop of modern agriculture. Journal of Future Foods, 1(1), 58-66. DOI: 10.1016/j.jfutfo.2021.09.001
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Stefan Gordanić, Ana Dragumilo, Snežana Mrđan, Jelena Golijan-Pantović, Sara Mikić, Željana Prijić, Tatjana Marković

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
